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    6-TAMRA Azide|6-TAMRA N3

    作者:乐虎lehu唯一官网 发布时间:2024-02-05 09:42:09 次浏览

    6-TAMRA Azide|6-TAMRA N3
    6-TAMRA Azide (also known as TAMRA azide, isomer 6) is the red-fluorescent probe that is patible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line. It is predominantly used for detection of terminal alkyne-tagged biomolecules via a copper-catalyzed click reaction (CUAAC). It also reacts with strained cyclooctyne via a copper-free &lduo;click chemistry&rduo; reaction to form a stable triazole and does not reuire Cu-catalyst or elevated temperatures.
    Although the mixed isomers of 5(6)-TAMRA azide is a preferred, routinely used red fluorescent dye for staining proteins, labeling peptides and nucleotides with TAMRA mixed isomers might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often reuired in the conjugation processes.
       
    6-TAMRA Azide|6-TAMRA N3
    Abs/Em Maxima
    553/575
    Extinction coefficient
    89,000
    Flow cytometry laser line
    532 or 555 nm
    Microscopy laser line
    532 or 555 nm
    Spectrally similar dyes
    Alexa Fluor® 546, Atto™ 543, CF™ 555 Dye, DyLight™549
    Molecular weight
    555.67
    CAS
    n/a
    Solubility
    Water, DMSO, DMF, MeOH
    Purity
    >98% (HPLC)
    Appearance
    Red solid
    Storage conditions
    -20C
    Shipping conditions
    Ambient temperature
    叠氮化合物(Azides)
    Azidobutyric acid NHS ester
    Azido dyes, available as solids, and as ready-to-use 10 mM DMSO solutions
    Water-soluble Cy3 azide
    Water-soluble Cy5 azide
    Cy3 azide
    Cy3.5 azide
    Cy5 azide
    Cy5.5 azide
    Cy7 azide
    Cy7.5 azide
    5-FAM azide
    6-FAM azide
    5-JOE azide
    5-R110 azide
    6-R110 azide
    6-ROX azide
    5-TAMRA azide
    炔烃类染料(Alkynes)
    Alkyne phosphoramidite, 5'-terminal
    Alkyne amidite, hydroxyprolinol
    Pentynoic acid STP ester
    荧光激活染料(Catalysts and activators)
    Ascorbic acid
    Copper(II)-TBTA plex, 10 mM in 55% a. DMSO
    SYBR Green I
    Gel staining solution, 10000x
    For PCR, 100x solution
    改良三磷酸盐(Modified triphosphates)
    Ami1-ddUTP
    Ami1-dUTP
    脂类活性染料(Dye activated esters)
    Cy3 NHS ester (water soluble)
    Cy5 NHS ester (water soluble)
    Cy3 NHS ester
    Cy3.5 NHS ester
    Cy5 NHS ester
    Cy5.5 NHS ester
    Cy7 NHS ester
    Cy7.5 NHS ester
    ROX NHS ester, pure 6- isomer
    羰基活性染料(Dye hydrazides)
    Cy5 hydrazide
    巯基反应性染料(Dye maleimides)
    Cy5 maleimide
    Cy5.5 maleimide
    2D DIGE荧光染料试剂盒(2D DIGE dye kits)
    Phosporamidites
    Alkyne phosphoramidite, 5'-terminal
    Alkyne amidite, hydroxyprolinol
    DMS(O)MT aminolink amidite
    Long trebler amidite
    Pyrene dU amidite
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